Newsroom
Imaging Through Tissue with Nanodiamonds
,

Our research on background free imaging through selective magnetic modulation of nanodiamond fluorescence was showcased on the cover of the April edition of Biomedical Optics Express. Currently, in vivo fluorescence-based imaging is hindered by background noise, especially because of autofluorescence that naturally occurs in tissue. Existing optical imaging methods thus have limitations in visualizing deep into tissue or with low concentrations of imaging probes.

Fluorescent nanodiamonds (FNDs) are biocompatible nanoparticles which have great potential both as probes and drug delivery agents. A wide-field magnetic modulation imaging system to eliminate background noise in FND emissions was developed. Its effectiveness was demonstrated with two methods by imaging FNDs overpowered by "background" dye under a microscope and in sentinel lymph nodes in mice. The first simply subtracts images with or without a magnetic field and further averages the subtracted images. This method could be implemented to create a running average image updated in real time. The second method, which relies on phase sensitive lock-in detection, can be implemented for smaller magnetic fields and faster modulation rates.

The closest lymph nodes to the tumor, sentinel nodes, are often a crucial indicator of the stage of cancer in the body. Using fluorescence imaging to detect sentinel lymph nodes is highly desired, but the technique suffers from background noise. After injecting FNDs into the foot pads of mice, images were taken to identify particles that drained to sentinel lymph nodes. Through magnetic modulation methods, lymph nodes were identified that were otherwise not visible with the conventional techniques of spectral unmixing. Magnetic modulation fluorescence imaging with nanodiamonds has demonstrated the potential to promote a more proactive and accurate diagnosis and prognosis of cancers.

Eliminating background noise is crucial to improving the efficacy and versatility of fluorescence imaging. By substantially increasing background rejection, the technique above not only improves existing FND based measurements but also has the potential to increase the tissue depth through which FNDs can be used in vivo, thus opening up new opportunities for this type of imaging.

Recent Posts
Star RA wins Research Award!
QB3 Invites Dr. Bumb to give a Lessons Learned Talk
Joyce Speaks at Nanobio
US Patent Granted
CareerGirls Feature Dr. Bumb
In the Community
Bikanta at the Molecular Foundry User Meeting
Jeanne Virca heads Bikanta's Sales Team
Ambika speaks on Nature Jobs Expo Panel
Bikanta Attends Cancer Moonshot Summit
Ambika speaks at Silicon Valley Boomer Competition
Ambika speaks at IEEE’s Nano Conference
A Value Added Materials Research Story
Georgia Tech honors Ambika with Alumni Award
Joyce dives into the Bikanta Team
Bikanta Team gives a live lab tour on Persicope
Ambika Bumb speaks on Nanoscience Panel
Bikanta Achieves 1st Place in Business Competition
Roger Energizes Bikanta's Instrumentation Team
A Nano Step For Man, A Giant Leap For Mankind
Bay Area’s Star Biotechs Present at FAST’s Showcase
Nanodiamonds Win the Orloff Award
WeFunder Features Bikanta
Ambika Joins Marshall Committee to Select Future Leaders
Collaborations with the National Institutes of Health
Bikanta goes to Capitol Hill
Bikanta Nominated for Diagnostic Startup of the Year
A Special Relationship: Scientific Training across the Atlantic
Siliconeer - Old is Gold
Bikanta Returns to Boomer Venture Summit
Min Wang Races onto Bikanta's Team
Ambika on Roominate’s #LetGirlsBuild
Introducing Bikanta's Newest Team Member, Li Zhuo
Bikanta’s CEO on CLSI’s BioGENEius Panel
Bikanta at the OneStart America
Vishesh Jain Joins the Team!
Jan 2015: Looking Back 6 Months
Bikanta's CEO Featured with 40 Female Founders
Bikanta on BBC's Tech Tent
Bikanta is Chosen for BayBio's FAST Program
Start-up Investor Y Combinator Invests in Bikanta
Winner of Silicon Valley Boomer Venture Competition
Bikanta's Boomer Win!
Home